JOVE JOURNAL

生物化学

Showing 361 - 372 in 776 videos.

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Karamat Mohammad¹, Heng Jiang², Vladimir I. Titorenko¹

¹Department of Biology,Concordia University, ²Centre for Biological Applications of Mass Spectrometry,Concordia University

Journal (Biochemistry)

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Chesley Marie Rowlett¹, Wenshe Ray Liu¹

¹Department of Chemistry,Texas A&M University

Journal (Biochemistry)

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Karidia Konate¹, Quentin Seisel¹, Eric Vivès¹, Prisca Boisguérin¹, Sébastien Deshayes¹

¹PHYMEDEXP,INSERM U1046 - CNRS UMR 9214 - University of Montpellier

Journal (Biochemistry)

Complementation of Splicing Activity by a Galectin-3 – U1 snRNP Complex on Beads

Patricia G. Voss¹, Kevin C. Haudek¹, Ronald J. Patterson², John L. Wang¹

¹Department of Biochemistry and Molecular Biology,Michigan State University, ²Department of Microbiology and Molecular Genetics,Michigan State University

Journal (Biochemistry)

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Roman Levin¹, Zoe Koeck¹, Volker Dötsch¹, Frank Bernhard¹

¹Institute of Biophysical Chemistry,Goethe University Frankfurt

Journal (Biochemistry)

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Sebastien Mailfert*¹, Karolina Wojtowicz*², Sophie Brustlein*¹, Ewa Blaszczak³, Nicolas Bertaux⁴, Marcin Łukaszewicz², Didier Marguet¹, Tomasz Trombik²

¹CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy,Aix Marseille Univ, ²Faculty of Biotechnology,University of Wroclaw, ³Faculty of Biological Sciences,University of Wroclaw, ⁴CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Journal (Biochemistry)

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Felipe A. Carvalho¹, Moises R. A. Barros¹, Thierry P. F. Girotto¹, M. Griselda Perona¹, Carla C. Oliveira¹

¹Department of Biochemistry, Institute of Chemistry,University of Sao Paulo

Journal (Biochemistry)

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Satish Bodakuntla*^1,2, A.S. Jijumon*^1,2, Carsten Janke^1,2, Maria M. Magiera^1,2

¹PSL Research University, CNRS UMR3348,Institut Curie, ²Université Paris-Saclay, CNRS UMR3348,Université Paris Sud

Journal (Biochemistry)

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Erin M. Anderson¹, Neil A. Greenwood¹, Dyanne Brewer², Cezar M. Khursigara¹

¹Department of Molecular and Cellular Biology,University of Guelph, ²Mass Spectrometry Facility,University of Guelph

Journal (Biochemistry)

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

Shannon K. D. Robin^1,2, Marc Ansari*^1,3, Chakradhara Rao S. Uppugunduri*^1,3

¹Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine,University of Geneva, ²Section of Biology, Faculty of Science,University of Geneva, ³Onco-Hematology Unit, Department of Women, Children-Adolescents,University Hospitals of Geneva

Journal (Biochemistry)

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Eduardo Hilario¹, Li Fan², Leonard J. Mueller¹, Michael F. Dunn²

¹Department of Chemistry,University of California-Riverside, ²Department of Biochemistry,University of California-Riverside

Journal (Biochemistry)

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Sayuri L. Higashi^1,2, Kazuya Yagyu^1,2, Haruna Nagase^1,2, Craig S. Pearson¹, Herbert M. Geller¹, Yasuhiro Katagiri¹

¹Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, ²United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University

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