JOVE JOURNAL

生物化学

Showing 325 - 336 in 776 videos.

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Journal (Biochemistry)

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Florence B Kineavy¹, Alex A Davies¹, Molly R Mitchell¹, Daniel Lay¹, Matt J Dominguez², Elliott J Stollar¹

¹School of Life Sciences,University of Liverpool, ²Health Sciences Center,Texas Tech University

Journal (Biochemistry)

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Danté Johnson¹, Benjamin Punshon-Smith², Jessica A. Espino¹, Anne Gershenson³, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences,University of Maryland Baltimore, ²Technology Research Center,University of Maryland Baltimore County, ³Department of Biochemistry and Molecular Biology,University of Massachusetts

Journal (Biochemistry)

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Misbha Ud Din Ahmad¹, Alexander Fish¹, Jeroen Molenaar¹, Sridhar Sreeramulu², Christian Richter², Nadide Altincekic², Harald Schwalbe², Hans Wienk¹, Anastassis Perrakis¹

¹Oncode Institute and Division of Biochemistry,the Netherlands Cancer Institute, ²Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ),Johann Wolfgang Goethe-University

Journal (Biochemistry)

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Licia E. Prestagiacomo¹, Caterina Gabriele¹, Paola Morelli², Maria Antonietta Rota³, Stefano Alba³, Giovanni Cuda¹, Rocco Damiano⁴, Marco Gaspari¹

¹Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine,Magna Graecia University of Catanzaro, ²Department of Health Science,Magna Graecia University of Catanzaro, ³Romolo Hospital, ⁴Department of Experimental and Clinical Medicine,Magna Graecia University of Catanzaro

Journal (Biochemistry)

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane (SSM)-Based Electrophysiology

Natalie Bärland¹, Camilo Perez¹

¹Biozentrum, University of Basel

Journal (Biochemistry)

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Ramona Duman¹, Christian M. Orr¹, Vitaliy Mykhaylyk¹, Kamel El Omari¹, Robert Pocock¹, Vinay Grama¹, Armin Wagner¹

¹Diamond Light Source Ltd, Harwell Science & Innovation Campus

Journal (Biochemistry)

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Eva Königshausen*¹, Christina Theresa Schmitz*¹, Lars Christian Rump¹, Lorenz Sellin¹

¹Department of Nephrology, Medical Faculty,Heinrich-Heine-University

Journal (Biochemistry)

¹⁵N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Aayushi Singh¹, Jeffrey A. Purslow¹, Vincenzo Venditti^1,2

¹Department of Chemistry,Iowa State University, ²Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Journal (Biochemistry)

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Miriam Yagüe-Capilla¹, Sean G. Rudd¹

¹Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet

Journal (Biochemistry)

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

Ivan V. Chicherin^1,2, Sergei A. Levitskii¹, Maria V. Baleva¹, Igor A. Krasheninnikov¹, Maxim V. Patrushev³, Piotr A. Kamenski¹

¹Department of Biology,M.V. Lomonosov Moscow State University, ²Institute of Functional Genomics,M.V. Lomonosov Moscow State University, ³National Research Centre "Kurchatov Institute"

Journal (Biochemistry)

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Lucia M. Q. Liao¹, Rachel A. V. Gray¹, Dale D. O. Martin¹

¹Department of Biology,University of Waterloo

Journal (Biochemistry)

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Natàlia de Martín Garrido¹, Kailash Ramlaul¹, Christopher H. S. Aylett¹

¹Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine,Imperial College London, South Kensington Campus

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