University at Buffalo View Institution's Website 14 articles published in JoVE Behavior Use of a Wireless Video-EEG System to Monitor Epileptiform Discharges Following Lateral Fluid-Percussion Induced Traumatic Brain Injury Matthew J. McGuire1, Steven M. Gertz1, Jolie D. McCutcheon2, Chelsea R. Richardson3, David J. Poulsen1 1Department of Neurosurgery, Jacobs School of Medicine and Biomedical Science, 2Comparative Medicine Laboratory Animal Facilities, University at Buffalo, 3emka Technologies Here we present a protocol to induce severe TBI with the lateral fluid percussion injury (FPI) model in adult, male Wistar rats. We also demonstrate the use of a wireless telemetry system to collect continuous video-EEG recordings and monitor for epileptiform discharges consistent with post-traumatic epileptogenesis. Behavior Short Session High Intensity Interval Training and Treadmill Assessment in Aged Mice Kenneth Ladd Seldeen1, Yonas Z. Redae1, Ramkumar Thiyagarajan1, Reem Nagi Berman1, Merced Marie Leiker1, Bruce Robert Troen1 1Division of Geriatrics and Palliative Medicine, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo and Research Service, Veterans Affairs Western New York Healthcare System Short session (≤10 min) high intensity interval training (HIIT) is emerging as an alternative to longer exercise modalities, yet the shorter variants are rarely modeled in animal studies. Here, we describe a 10 min, 3 day a week, uphill treadmill HIIT protocol that enhances physical performance in male and female aged mice. Immunology and Infection Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System Andrew D. McCall1, Mira Edgerton1 1Department of Oral Biology, University at Buffalo We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images. Medicine The Perinatal Asphyxiated Lamb Model: A Model for Newborn Resuscitation Payam Vali*1, Sylvia Gugino*2, Carmon Koenigsknecht2, Justin Helman2, Praveen Chandrasekharan2, Munmun Rawat2, Satyan Lakshminrusimha1, Jayasree Nair2 1Department of Pediatrics, University of California Davis, 2Department of Pediatrics, University at Buffalo Invasive instrumentation of the fetal lamb provides accurate physiologic measurements of the transitioning circulation in a model that closely mimics the newly born infant. Bioengineering Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification Thien N. Tran1, Sankara N. Ramanan1, Haiqing Lin1 1Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York at Buffalo This paper reports practical methods to prepare hydrogels in freestanding films and impregnated membranes and to characterize their physical properties, including water transport properties. Behavior Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats Robert J. Wickham1, Jinwoo Park2, Eric J. Nunes3, Nii A. Addy1,3,4 1Interdepartmental Neuroscience Program, Yale University, 2Department of Biotechnical and Clinical Laboratory Sciences, School of Medicine and Biomedical Sciences, University at Buffalo, 3Department of Psychiatry, Yale School of Medicine, 4Department of Cellular and Molecular Physiology, Yale School of Medicine Rapid fluctuations in extracellular dopamine (DA) mediate both reward processing and motivated behavior in mammals. This manuscript describes the combined use of fast scan cyclic voltammetry (FSCV) and intra-oral tastant administration to determine how tastants alter rapid dopamine release in awake, freely moving rats. Immunology and Infection Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy Melissa J. Grimm1, Anthony C. D'Auria1, Brahm H. Segal2 1Department of Medicine, Roswell Park Cancer Institute, 2Department of Medicine, School of Medicine and Biomedical Sciences, University of Buffalo A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy. Biology The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli Ming Jiang1, Haoran Zhang2, Blaine A. Pfeifer1 1Chemical and Biological Engineering Department, State University of New York at Buffalo, 2Chemical Engineering Department, Massachusetts Institute of Technology The heterologous biosynthesis of erythromycin A through E. coli includes the following experimental steps: 1) genetic transfer; 2) heterologous reconstitution; and 3) product analysis. Each step will be explained in the context of the motivation, potential, and challenges in producing therapeutic natural products using E. coli as a surrogate host. Immunology and Infection Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models Wei Han1, Hui Li1, Brahm H. Segal2,3, Timothy S. Blackwell1 1Department of Medicine, Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology, Roswell Park Cancer Institute, 3Department of Medicine, University at Buffalo School of Medicine NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described. Biology Electricity-Free, Sequential Nucleic Acid and Protein Isolation David R. Pawlowski1,2, Richard J. Karalus1,2 1CUBRC, Inc., 2Department of Microbiology and Immunology, State University of New York at Buffalo, School of Medicine and Biomedical Sciences A tool and chemistries are described to sequentially isolate nucleic acids followed by proteins from a sample without the need for electricity. The tool consists of a sorbent held within a transfer pipette while the isolation chemistries are based on solid-phase extraction principles. The isolated macromolecules can be analyzed by immuno-based and PCR-based assays. Neuroscience Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos Tadeusz J. Kaczynski1, Shermali Gunawardena1 1Department of Biological Sciences, SUNY-University at Buffalo We describe the procedure to prepare staged Drosophila embryos for the visualization of the embryonic nervous system during embryogenesis. Neuroscience In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons Michelle L. Kuznicki1, Shermali Gunawardena1 1Department of Biological Sciences, SUNY-University at Buffalo This protocol discusses the live dissection of Drosophila larvae for the purpose of imaging the movement of GFP tagged axonal vesicles on microtubule tracks. Neuroscience Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations Samantha Fye1, Kunsang Dolma1, Min Jung Kang1, Shermali Gunawardena1 1Department of Biological Sciences, SUNY-University at Buffalo Drosophila melanogaster larvae provide an ideal model system to investigate the mechanisms of axonal transport within larval segmental nerves. Using this procedure, 3rd instar larvae carrying various mutations can be compared to wild type larvae. Biology In situ Protocol for Butterfly Pupal Wings Using Riboprobes Diane Ramos1, Antonia Monteiro2 1Department of Biological Sciences, SUNY-University at Buffalo, 2Dept. Ecology and Evolutionary Biology, Yale University In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.