University of Nevada, Reno View Institution's Website 10 articles published in JoVE Neuroscience Measurement of Poly A Tail Length from Drosophila Larva Brain and Cell Line Monika Singh1, Jung Hwan Kim1 1Department of Biology, University of Nevada, Reno The protocol describes an efficient and reliable method for quantifying the poly(A) length of the gene of interest from the Drosophila nervous system, which can be easily adapted to tissues or cell types from other species. Biochemistry Formulation and Characterization of Bioactive Agent Containing Nanodisks Kyle Lethcoe1, Colin A. Fox1, Isabel Moh1, Matthew Swackhamer1, Michael Karo1, Ryan Lockhart1, Robert O. Ryan1 1Department of Biochemistry and Molecular Biology, University of Nevada Here, we describe the production and characterization of bioactive agents containing nanodisks. Amphotericin B nanodisks are taken as an example to describe the protocol in a stepwise manner. Biology Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis Arvind Sharma1, Michael Pham1, Robert A. Harrell II2, Andrew B. Nuss3, Monika Gulia-Nuss1 1Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 2Insect Transformation Facility, University of Maryland Institute for Bioscience and Biotechnology Research, 3Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno The present protocol describes a method for injecting tick embryos. Embryo injection is the preferred technique for genetic manipulation to generate transgenic lines. Behavior Methods for Presenting Real-world Objects Under Controlled Laboratory Conditions Carissa A. Romero1, Jacqueline C. Snow1 1Department of Psychology, University of Nevada, Reno We describe methods for presenting real-world objects and matched images of the same objects under tightly-controlled experimental conditions. The methods are described in the context of a decision-making task, but the same real-world approach can be extended to other cognitive domains such as perception, attention, and memory. Neuroscience Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm Dante J. Heredia1, Grant W. Hennig2, Thomas W. Gould1 1Department of Physiology and Cell Biology, School of Medicine, University of Nevada, 2Department of Pharmacology, Larner College of Medicine, University of Vermont Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction. Neuroscience Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons David A. Clark1,2, Donovan Kohler1, America Mathis1, Eryn Slankster1, Samipya Kafle1, Seth R. Odell1,2, Dennis Mathew1,2 1Department of Biology, MS-0314, University of Nevada, 2Integrated Neuroscience Graduate Program, University of Nevada This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software. Immunology and Infection Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice Andrew B. Nuss1, Manoj G. Mathew2, Monika Gulia-Nuss2 1Department of Agriculture, Nutrition, and Veterinary Sciences, University of Nevada, Reno, 2Department of Biochemistry and Molecular Biology, University of Nevada, Reno The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice. Behavior Visualizing Visual Adaptation Michael A. Webster1, Katherine E.M. Tregillus1 1Department of Psychology, University of Nevada, Reno This article describes a novel method for simulating and studying adaptation in the visual system. Behavior Task Interruption and Resumption Paradigm for Testing the Activation and Pursuit of an Abstract Thinking Goal Jae-Eun Namkoong1, Marlone D. Henderson2 1Managerial Sciences Department, University of Nevada, 2Department of Psychology, University of Texas This protocol was designed to test the activation and pursuit of cognitive goals (e.g., an abstract thinking goal) using the task interruption and resumption paradigm. The protocol is suitable for cognitive goals that are automatically pursued once activated, as the distraction procedure prevents goal pursuit during the interruption period. Medicine A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis Michele M. Pelter1, Teri M. Kozik2, Denise L. Loranger1, Mary G. Carey3 1Orvis School of Nursing, University of Nevada, Reno, 2 Continuous 12-lead electrocardiographic (ECG) monitoring can identify transient myocardial ischemia, even when asymptomatic, among patients with suspected acute coronary syndrome (ACS). In this article we describe our method for initiating patient monitoring using a Holter device, downloading the ECG data for off-line analysis, and how to utilize the ECG software to identify transient ischemia.
