Johns Hopkins Whiting School of Engineering 1 article published in JoVE Biology Guinea Pig Round Window Membrane Explantation for Ex Vivo Studies Sarek A. Shen1, Mukund Madhav Goyal2, Kelly Lane1, Mohamed Lehar1, Daniel Q. Sun1,3 1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins School of Medicine, 2Department of Chemical and Biomolecular Engineering, Johns Hopkins Whiting School of Engineering, 3Department of Otolaryngology-Head and Neck Surgery, University of Cincinnati College of Medicine This protocol outlines a method for the explantation of the round window membrane from guinea pig temporal bones, providing a valuable resource for ex vivo studies.