Department of Energy 1 article published in JoVE Environment Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions Jian Gao1,2, Joelle Sasse1,2, Kyle M. Lewald1,2, Kateryna Zhalnina1,2, Lloyd T. Cornmesser1,2, Todd A. Duncombe3, Yasuo Yoshikuni2, John P. Vogel2, Mary K. Firestone4, Trent R. Northen1,2 1Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 2Joint Genome Institute, Department of Energy, 3Joint BioEnergy Institute, 4Department of Environmental Science Policy and Management, University of California This article describes detailed protocols for ecosystem fabrication of devices (EcoFABs) that enable the studies of plants and plant-microbe interactions in highly controlled laboratory conditions.