JOVE JOURNAL

生物化学

Showing 373 - 384 in 731 videos.

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Journal (Biochemistry)

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Yibing Deng, Bin Zheng, Yutong Liu, Shengchao Shi, Jingyuan Nie, Tao Wu, Peng Zheng

¹State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Journal (Biochemistry)

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Jillian Rosenberg, Jun Huang²

¹Committee on Cancer Biology,The University of Chicago, ²Pritzker School of Molecular Engineering,The University of Chicago

Journal (Biochemistry)

A Rapidly Incremented Tethered-Swimming Maximal Protocol for Cardiorespiratory Assessment of Swimmers

Dalton M. Pessôa Filho*^1,2, Danilo A. Massini*², Leandro O. C. Siqueira*², Luiz Gustavo A. Santos*², Camila M. T. Vasconcelos*², Tiago A. F. Almeida*^1,3, Mário A. C. Espada*^4,5, Joana F. Reis*^3,6, Francisco B. Alves*³, Fred J. DiMenna*^7,8

¹Department of Physical Education,São Paulo State University (UNESP) at Bauru, ²Institute of Bioscience, Graduate Program in Human Development and Technology,São Paulo State University (UNESP) at Rio Claro, ³Ciper, Faculdade de Motricidade Humana,Universidade de Lisboa, ⁴Department of Science and Technology, School of Education,Polytechnic Institute of Setúbal, ⁵Quality of Life Research Center,Polytechnic Institute of Santarem, ⁶Universidade Europeia at Lisbon, ⁷Division of Endocrinology, Diabetes and Bone Disease,Icahn School of Medicine at Mount Sinai, ⁸Department of Biobehavioral Sciences, Teachers College,Columbia University

Journal (Biochemistry)

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Melanie Balbach, Jochen Buck, Lonny R. Levin

¹Department of Pharmacology,Weill Cornell Medical College

Journal (Biochemistry)

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Parnian Bigdelou, Amir M. Farnoud²

¹Biomedical Engineering Program,Ohio University, ²Department of Chemical and Biomolecular Engineering,Ohio University

Journal (Biochemistry)

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Constance Agamasu, Peter Frank, Shelley Perkins, Timothy Waybright, Simon Messing, William Gillette, Andrew G. Stephen

¹NCI RAS Initiative, Cancer Research Technology Program,Frederick National Laboratory for Cancer Research

Journal (Biochemistry)

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Ping Wei*¹, Qihong Liu*², Wen Xue, Jiwu Wang

¹Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People's Hospital, ²Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Journal (Biochemistry)

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Luis Gomez-Castillo*¹, Kurumi Watanabe*¹, Huayi Jiang*¹, Shoukai Kang, Liangcai Gu

¹Department of Biochemistry and Institute for Protein Design,University of Washington

Journal (Biochemistry)

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Ahmed Ali², Yasmine Abouleila², Arno Germond

¹Biodynamics Research Center (BDR), RIKEN, ²Research Center,Misr International University

Journal (Biochemistry)

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Christine Terryn, Anouck Habrant, Gabriel Paës, Corentin Spriet

¹PICT Platform,Université de Reims Champagne-Ardenne, ²Fractionation of AgroResources and Environment (FARE) Laboratory, INRA,Université de Reims Champagne-Ardenne, ³Unité de Glycobiologie Structurale et Fonctionnelle,Université de Lille

Journal (Biochemistry)

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Menaka Ariyaratne, Lingxiao Ge, Paul F. Morris

¹Department of Biological Sciences,Bowling Green State University

Journal (Biochemistry)

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Adam H. Barczewski, Michael J. Ragusa, Dale F. Mierke, Maria Pellegrini

¹Department of Chemistry,Dartmouth College

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