1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Apresentamos um protocolo para montagem modular baseada em CRISPR (CRISPRmass), um método para construção de alto rendimento da biblioteca de plasmídeos UAS-cDNA/ORF em Drosophila usando recursos de cDNA/ORF disponíveis publicamente. O CRISPRmass pode ser aplicado à edição de várias bibliotecas de plasmídeos.
