Journal
生物化学
cAMP Analysis with cADDis: Single-Cell to High-Throughput Screening

cAMP Analysis with cADDis: Single-Cell to High-Throughput Screening

March 22, 2024

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

01:20 min
•
391 Views

Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy

02:14 min
•
71 Views

Inverted Fluorescence Microscopy and Data Analysis to Assess Drug Effects on cAMP in HASM Cells

02:33 min
•
54 Views
, , , ,
1Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Summary

The protocol presented in this study illustrates the effectiveness of the cAMP Difference Detector In Situ in measuring cAMP through two methods. One method utilizes a 96-well plate reading spectrophotometer with HEK-293 cells. The other method demonstrates individual HASM cells under a fluorescent microscope.

Article