Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Miriam Wandres; Denise Aigner; Nicolai Kastelic; Anastasiya Boltengagen; Agnieszka Rybak-Wolf; Nikolaus Rajewsky

doi:10.3791/66225

Journal

发育生物学

Single Cell and Nuclei Extraction from Human Brain Organoids

March 29, 2024

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

02:19 min

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951 Views

Isolation of Single Cells from Human Brain Organoids for Single-Cell RNA Sequencing

01:38 min

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63 Views

Isolation of Single Nuclei from Human Brain Organoids for Single-Nucleus RNA Sequencing

01:55 min

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64 Views

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic^1,3, Anastasiya Boltengagen¹, Agnieszka Rybak-Wolf^1,3, Nikolaus Rajewsky^1,2

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

Summary

Here, we introduce a comprehensive protocol for the generation and downstream analysis of human brain organoids using single-cell and single-nucleus RNA sequencing.

Single Cell and Nuclei Extraction from Human Brain Organoids

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Isolation of Single Cells from Human Brain Organoids for Single-Cell RNA Sequencing

Isolation of Single Nuclei from Human Brain Organoids for Single-Nucleus RNA Sequencing

Summary

Article