Sample Preparation for <em>In Situ</em> Cryotomography of Mammalian Cells

Noah Weber; Brennan Hinks; Jacob Jensen; Thomas Lidahl; Luiza Mendon&#231;a

doi:10.3791/65697

Journal

生物化学

Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

December 15, 2023

Author Spotlight: Optimizing Grid Preparation for Enhanced Cryoelectron Tomography

01:19 min

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1,240 Views

Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography

02:29 min

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305 Views

Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography

02:04 min

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321 Views

Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography

02:45 min

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222 Views

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Summary

This method provides an accessible and flexible protocol for the preparation of electron microscopy (EM) grids for in situ cellular cryotomography and correlative light and electron microscopy (CLEM).