Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

Am&#233;lie Weiss; Peter Sommer; Johannes H. Wilbertz

doi:10.3791/65570

Journal

神经科学

Culturing Human Midbrain Dopaminergic Neurons for Phenotypic Profiling

July 07, 2023

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

02:16 min

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907 Views

Thawing Human iPSC-Derived mDA Neurons for Seeding

02:23 min

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150 Views

Human iPSC-Derived mDA Neurons Seeding and Compound Treatment for Phenotypic Profiling

02:37 min

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86 Views

Image Processing of Fluorescently-Stained Human iPSC-Derived mDA Neurons for Phenotypic Profile Generation

02:32 min

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89 Views

Amélie Weiss¹, Peter Sommer¹, Johannes H. Wilbertz¹

¹Ksilink

Summary

This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical modulations.