Isolating Small Extracellular Vesicles and Extracting Their Peptidome

Isolating Small Extracellular Vesicles and Extracting Their Peptidome

June 30, 2023

从骨髓来源的巨噬细胞中鉴定小细胞外囊泡的肽

通过差示超速离心分离小细胞外囊泡（sEV）

从分离的小细胞外囊泡（sEV）中提取肽

Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

Summary

该协议描述了通过差异超速离心从巨噬细胞中分离小细胞外囊泡并提取肽圆顶以进行质谱鉴定的程序。

Article