Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Lingzhi Fan; Yue Luo; Wannian Yan; Huanxing Han; Pengfei Zhang

doi:10.3791/67000

Journal

Biochemistry

Quantum Dot Nanobeads for Enhanced Sensitivity in Biomarker Detection

June 28, 2024

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

01:57 min

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186 Views

Preparation of Quantum Dots (QDs) Nanobeads for QDNB-Based Lateral Flow Immunoassay

01:11 min

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46 Views

QDNB-Antibody Conjugates Preparation Using One-Step Emulsion Evaporation Method

01:48 min

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40 Views

Lateral Flow Immunoassay Strips Preparation, Assay Operation and its Quantitative Evaluation

02:29 min

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77 Views

Lingzhi Fan*¹, Yue Luo*², Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine,Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine,Tongji University, ³Department of Pharmacy, Changzheng Hospital,Naval Medical University

Summary

Here, we describe a protocol for the preparation of quantum dot nanobeads (QDNB) and the detection of disease biomarkers using QDNB-based lateral flow immunoassay strips. The test results can be qualitatively assessed under UV light illumination and quantitatively measured using a fluorescent strip reader within 15 min.