1John T. Milliken Department of Medicine, Division of Nutritional Sciences and Obesity Medicine,Washington University School of Medicine, 2Nutrition and Obesity Research Center, Cellular and Molecular Biology Core,Washington University School of Medicine, 3Nutrition and Obesity Research Center, Animal Model Research Core,Washington University School of Medicine
Here, we describe a comprehensive method for measuring mitochondrial oxidative phosphorylation in fresh permeabilized skeletal muscle fibers from either human or mouse muscle. This method allows for the real-time quantification of mitochondrial respiration and the assessment of fuel preference and metabolic flexibility while preserving existing mitochondrial networks and membrane integrity.
