Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Aline Linder; Kevin Portmann; Luca Johannes Schlotheuber; Alessandro Streuli; Wiona Sophie Gl&#228;nzer; Klaus Eyer; Ines L&#252;chtefeld

doi:10.3791/66492

Journal

Immunology and Infection

Microfluidic Droplet Assay for Single-Cell Cytokine Secretion Analysis

March 08, 2024

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

02:07 min

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385 Views

Observation Chamber Fabrication for Imaging Single-Cell Suspensions and Magnetic Nanoparticles

01:50 min

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117 Views

Preparation of Functionalized Magnetic Nanoparticles for Time-Resolved Fluorescent Imaging

01:47 min

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100 Views

Encapsulation of the Cells and Nanoparticles into Droplets for Fluorescent Imaging

04:11 min

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91 Views

Aline Linder*¹, Kevin Portmann*¹, Luca Johannes Schlotheuber¹, Alessandro Streuli¹, Wiona Sophie Glänzer¹, Klaus Eyer^1,2, Ines Lüchtefeld^1,3

¹Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences,ETH Zürich, ²Department of Biomedicine,Aarhus University, ³Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology,ETH Zürich

Summary

The protocol describes an advanced microfluidic platform to quantitatively measure cytokine secretion dynamics of individual human peripheral blood mononuclear cells. The platform measures up to three cytokines in parallel (IL-6, TNFα, and IL-1β) for each individual cell stimulated with lipopolysaccharide as an example.