JoVE
JoVE
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Faculty Resource Center
Authors
Solutions
For Libraries
For Higher Education Courses
For High Schools
Contact Us
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
JoVE High schools
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
Biology
Crude Vector Production and Efficient DNA Delivery in Cell Cultures
Crude Vector Production and Efficient DNA Delivery in Cell Cultures
October 20, 2023
使用未纯化的重组腺相关病毒载体在培养细胞中进行转基因表达
01:49 min
•
1,322 Views
重组腺相关病毒载体的生产和培养细胞的转导
05:10 min
•
420 Views
Brian Benyamini
1
,
Meagan N. Esbin
1,2
,
Oscar Whitney
1
,
Nike Walther
1
,
Anna C. Maurer
1
1
Department of Molecular and Cell Biology
,
University of California, Berkeley
,
2
Biophysics Graduate Group
,
University of California, Berkeley
Summary
English
English (Original)
العربية (Arabic)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatically Generated
重组腺相关病毒（rAAV）广泛用于临床和临床前基因递送。rAAV的一个被低估的用途是培养细胞的稳健转导，无需纯化。对于刚接触rAAV的研究人员，我们提供了转基因盒克隆、粗载体生产和细胞培养转导的方案。
Article
