Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

June 23, 2023

大鼠肠道类器官的产生和操作

小肠类器官的建立和传代

从3D类器官生成大鼠肠道2D单层

Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen^3,4, Kaelyn Sumigray^1,5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

Summary

在这里，我们提出了一种产生大鼠肠道类器官并将其用于几种下游应用的方案。大鼠通常是首选的临床前模型，强大的肠道类器官系统满足了对体外系统伴随体内研究的需求。

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