JOVE JOURNAL

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Assaying for Inorganic Polyphosphate in Bacteria

Arya Pokhrel¹, Jordan C. Lingo², Frank Wolschendorf², Michael J. Gray¹

¹Department of Microbiology,University of Alabama at Birmingham, ²Division of Infectious Diseases,University of Alabama at Birmingham

Journal (Immunology and Infection)

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Michel Jourdan¹, Hugues de Boussac¹, Elena Viziteu¹, Alboukadel Kassambara¹, Jérôme Moreaux^1,2,3

¹IGH, CNRS,Univ Montpellier, ²Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology,CHU Montpellier, ³UFR de Médecine,Univ Montpellier

Journal (Immunology and Infection)

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Katherine Schultheis¹, Hubert Schaefer², Holly M. Pugh¹, Bryan S. Yung¹, Janet Oh¹, Jacklyn Nguyen¹, Laurent Humeau¹, Kate E. Broderick¹, Trevor R.F. Smith¹

¹Inovio Pharmaceuticals, Inc, ²Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Journal (Immunology and Infection)

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Sultan Asad^1,2, Fabiana Feitosa-Suntheimer^1,2, Alexander Gold^1,2, Berlin Londono-Renteria², Tonya M. Colpitts^1,2

¹Department of Microbiology,Boston University School of Medicine, ²National Emerging Infectious Diseases Laborator,Boston University School of Medicine, ³Entomoloji Anabilim Dalı,Kansas State University

Journal (Immunology and Infection)

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

John-William Sidhom^1,2,3, Debebe Theodros^1,2,4, Benjamin Murter^1,2, Jelani C. Zarif^1,2, Sudipto Ganguly^1,2, Drew M. Pardoll^1,2, Alexander Baras^1,2,5

¹The Bloomberg~Kimmel Institute for Cancer Immunotherapy,Johns Hopkins University School of Medicine, ²The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, ³Department of Biomedical Engineering,Johns Hopkins University School of Medicine, ⁴Department of Immunology,Johns Hopkins University School of Medicine, ⁵Patoloji Bölümü,Johns Hopkins University School of Medicine

Journal (Immunology and Infection)

Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy

Liangfei Niu¹, Cong Wang¹, Kaile Zhang^1,2, Miaomiao Kang^1,2, Rui Liang¹, Xiaonan Zhang¹, Bo Yan¹

¹Shanghai Public Health Clinical Center,Fudan University, ²School of Life Sciences,Bengbu Medical College

Journal (Immunology and Infection)

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Chin-Wen Wei¹, Tyng-An Zhou¹, Ivan L. Dzhagalov¹, Chia-Lin Hsu¹

¹Institute of Microbiology and Immunology,National Yang-Ming University

Journal (Immunology and Infection)

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Guo Hong*^1,2, Shuangyi Fan*³, The Phyu³, Priyanka Maheshwari², Michal Marek Hoppe², Hoang Mai Phuong², Sanjay de Mel⁴, Michelle Poon⁴, Siok-Bian Ng^2,3, Anand D. Jeyasekharan^2,4

¹Department of Laboratory Medicine,AnSteel Group General Hospital, ²Cancer Science Institute of Singapore,National University of Singapore, ³Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, ⁴Department of Haematology-Oncology,National University Health System

Journal (Immunology and Infection)

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Jonathan G.T. Lam^1,2,3, Chi Song⁴, Stephanie Seveau^1,2,3

¹Department of Microbial Infection and Immunity,The Ohio State University, ²Department of Microbiology,The Ohio State University, ³Infectious Diseases Institute,The Ohio State University, ⁴Division of Biostatistics, College of Public Health,The Ohio State University

Journal (Immunology and Infection)

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Jeffrey M. Finlon¹, Matthew A. Burchill¹, Beth A. Jirón Tamburini^1,2

¹Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, ²Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Journal (Immunology and Infection)

Using the Protozoan Paramecium caudatum as a Vehicle for Food-borne Infections in Zebrafish Larvae

Erika Flores¹, Laurel Thompson¹, Natalie Sirisaengtaksin¹, Anh Trinh Nguyen¹, Abigail Ballard¹, Anne-Marie Krachler¹

¹McGovern Medical School, Department of Microbiology and Molecular Genetics,University of Texas Health Science Center at Houston

Journal (Immunology and Infection)

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Xin Xia¹, Haitang Li¹, Sangeetha Satheesan^1,2, Jiehua Zhou¹, John J. Rossi¹

¹Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, ²Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

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