JOVE JOURNAL

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An Easy Method for Plant Polysome Profiling

Cécile Lecampion^1,2,3, Maïna Floris^1,2,3,4, Jean Raphaël Fantino^5,6, Christophe Robaglia^1,2,3, Christophe Laloi^1,2,3

¹Laboratoire de Génétique et Biophysique des Plantes,Aix-Marseille Université, ²UMR 7265 Biologie Végétale & Microbiologie Environnementales,CNRS, ³BIAM,CEA, ⁴Department of Biology, Biocenter,University of Copenhagen, ⁵Laboratoire de Chimie Bactérienne, ⁶CNRS, LCB UMR 7283,Aix Marseille Université

Journal (Biology)

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

Justin Judd¹, Jonathan Lovas¹, Guo N. Huang¹

¹Cardiovascular Research Institute,University of California, San Francisco

Journal (Biology)

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Chelsea S. Rule¹, Marcella Patrick¹, Maria Sandkvist¹

¹Department of Microbiology and Immunology,University of Michigan

Journal (Biology)

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Karla A. Mettrick¹, Nikki Lawrence¹, Claire Mason¹, Georgia M. Weaver¹, Tayla-Ann Corocher¹, Ian Grainge¹

¹School of Environmental and Life Sciences,University of Newcastle

Journal (Biology)

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Palak G. Patel^1,2, Shamini Selvarajah^1,2, Suzanne Boursalie^1,2, Nathan E. How^1,2, Joshua Ejdelman³, Karl-Philippe Guerard³, John M. Bartlett⁴, Jacques Lapointe³, Paul C. Park¹, John B. A. Okello^1,2, David M. Berman^1,2

¹Department of Pathology & Molecular Medicine,Queen's University, ²Division of Cancer Biology & Genetics, Queen's Cancer Research Institute,Queen's University, ³Department of Surgery, Division of Urology,McGill University, ⁴Transformative Pathology Program,Ontario Institute for Cancer Research (OICR)

Journal (Biology)

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Daniel E. Miller¹, Zubin H. Patel^1,2,3, Xiaoming Lu^1,3, Arthur T. Lynch¹, Matthew T. Weirauch*^1,4, Leah C. Kottyan*¹

¹Center for Autoimmune Genomics and Etiology,Cincinnati Children's Hospital, ²Medical Scientist Training Program,University of Cincinnati, ³Immunology Graduate Program,University of Cincinnati, ⁴Divisions of Biomedical Informatics and Developmental Biology,Cincinnati Children's Hospital

Journal (Biology)

Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry

Abdulkadir Abakir¹, Lee Wheldon², Andrew D. Johnson³, Patrick Laurent¹, Alexey Ruzov⁴

¹Laboratoire de Neurophysiologie (CP601), ULB Neuroscience Institute (UNI),Université Libre de Bruxelles, ²Medical Molecular Sciences, Centre for Biomolecular Sciences,University of Nottingham, ³School of Life Sciences,University of Nottingham, ⁴Division of Cancer and Stem Cells, Centre for Biomolecular Sciences, School of Medicine,University of Nottingham

Journal (Biology)

Rodent Working Heart Model for the Study of Myocardial Performance and Oxygen Consumption

Elizabeth S. DeWitt*¹, Katherine J. Black*¹, John N. Kheir¹

¹Department of Cardiology,Boston Children’s Hospital

Journal (Biology)

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Michael Urban¹, Marc Vor der Brüggen², Robert Tampé¹

¹Institute of Biochemistry, Biocenter,Goethe University Frankfurt, ²Nanospot GmbH

Journal (Biology)

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Mathijs Vleugel¹, Sophie Roth¹, Celebrity F. Groenendijk¹, Marileen Dogterom¹

¹Department of Bionanoscience,Delft University of Technology

Journal (Biology)

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Alexander Braun¹, Nicole M. Caesar¹, Kyvan Dang¹, Kenneth A. Myers¹

¹Department of Biological Sciences,University of the Sciences in Philadelphia

Journal (Biology)

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts

Johannes F. Buyel^1,2, Jürgen Hubbuch³, Rainer Fischer^1,2

¹Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., ²Institute for Molecular Biotechnology,RWTH Aachen University, ³Department of Biomolecular Separation Engineering,Karlsruhe Institute of Technology (KIT)

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