JOVE JOURNAL

Biyomühendislik

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Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Kleopatra Rapti^1,2, Olena Maiakovska^1,2, Jonas Becker^1,2, Joanna Szumska^1,2, Margarita Zayas^1,2, Felix Bubeck^1,2, Jixin Liu^1,2, Emma Gerstmann^1,2, Chiara Krämer^1,2, Ellen Wiedtke^1,2, Dirk Grimm^1,2,3,4,5

¹Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty,University of Heidelberg, ²BioQuant Center, BQ0030,University of Heidelberg, ³Cluster of Excellence CellNetworks, ⁴German Center for Infection Research (DZIF), ⁵German Center for Cardiovascular Research (DZHK)

Journal (Bioengineering)

Investigating Stress-relaxation and Failure Responses in the Trachea

Anita Singh¹, Tanmay Majmudar^2,3, Adi Iyer⁴, Diya Iyer⁴, Sriram Balasubramanian³

¹Department of Biomedical Engineering,Widener University, ²Drexel University College of Medicine, ³School of Biomedical Engineering, Science and Health Systems,Drexel University, ⁴Rosetree Media School District

Journal (Bioengineering)

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

Prathibha Yarikipati¹, Andreas Bergmann¹

¹Department of Molecular, Cell and Cancer Biology,UMass Chan Medical School

Journal (Bioengineering)

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Albert M. Liao¹, Tran Thi Thanh Thoa¹, G. Thomas Caltagirone¹

¹Aptagen LLC

Journal (Bioengineering)

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Paula M. Cevaal¹, Michael Roche², Sharon R. Lewin^2,3,4, Frank Caruso⁵, Matthew Faria⁶

¹Department of Microbiology and Immunology,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, ²Department of Infectious Diseases,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, ³Victorian Infectious Diseases Service,Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, ⁴Department of Infectious Diseases,Alfred Hospital and Monash University, ⁵Department of Chemical Engineering,The University of Melbourne, ⁶Department of Biomedical Engineering,The University of Melbourne

Journal (Bioengineering)

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Madelyn VanBlunk¹, Pritha Agarwalla^1,2, Sharda Pandit^1,2, Yevgeny Brudno^1,2,3

¹Joint Department of Biomedical Engineering,University of North Carolina at Chapel Hill and North Carolina State University, ²Comparative Medicine Institute,North Carolina State University, ³Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill

Journal (Bioengineering)

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Adeel Ahmed¹, Indranil M. Joshi¹, Madeleine R. Goulet¹, Justin A. Vidas¹, Ann M. Byerley¹, Mehran Mansouri¹, Steven W. Day¹, Vinay V. Abhyankar¹

¹Department of Biomedical Engineering, Kate Gleason College of Engineering,Rochester Institute of Technology

Journal (Bioengineering)

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Marcos Ezequiel Gramajo*¹, Ryan J. Lake*², Yi Lu³, Ana Sol Peinetti¹

¹INQUIMAE (CONICET), Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires, ²Kimya Bölümü,University of Illinois at Urbana-Champaign, ³Kimya Bölümü,University of Texas at Austin

Journal (Bioengineering)

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Fengqiang Cao*^1,2,3, Yali Ming*^3,4, Weixiang Gao^3,4, Jia Ge³, Kenji Ogino^1,2

¹Graduate School of Bio-Applications and Systems Engineering,Tokyo University of Agriculture and Technology, ²Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, ³State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,Chinese Academy of Sciences, ⁴University of Chinese Academy of Sciences

Journal (Bioengineering)

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Yuhao Yuan¹, Fake Lu¹

¹Department of Biomedical Engineering, Binghamton University,State University of New York

Journal (Bioengineering)

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Sheng Chen¹, Zachary Gao Sun^2,3, Michael P. Murrell^1,2,3

¹Department of Biomedical Engineering,Yale University, ²Systems Biology Institute,Yale University, ³Fizik Bölümü,Yale University

Journal (Bioengineering)

Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

Elena Gracheva¹, Fei Wang¹, Abigail Matt¹, Hongwu Liang¹, Matthew Fishman^1,2, Chao Zhou¹

¹Department of Biomedical Engineering,Washington University in St. Louis, ²Department of Computer Science and Engineering,Washington University in St. Louis

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