JOVE JOURNAL

Genetics

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Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Yassemine Khawajkie¹, Nawel Mechtouf², Phuong Nguyen², Rima Slim^1,2,3

¹Department of Experimental Medicine,McGill University, ²Department of Human Genetics,McGill University, ³Department of Obstetrics and Gynecology,McGill University

Journal (Genetics)

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Shao-Yen Kao*¹, Elena Nikonova*¹, Keshika Ravichandran*¹, Maria L. Spletter^1,2

¹Biomedical Center, Department of Physiological Chemistry,Ludwig-Maximilians-University Munich, ²Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry,Ludwig-Maximilians-University Munich

Journal (Genetics)

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Vivek Bhaskaran¹, Pierpaolo Peruzzi¹

¹Department of Neurosurgery,Brigham and Women's Hospital-Harvard Medical School

Journal (Genetics)

A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TE^deff) in Cancers

Vishnu Modur¹, Navneet Singh¹, Belal Muhammad¹

¹Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute,Cincinnati Children's Hospital Medical Center (CCHMC)

Journal (Genetics)

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Fabian L. Cardenas-Diaz*¹, Jean Ann Maguire*¹, Paul Gadue^1,2,3, Deborah L. French^1,2,3

¹Center for Cellular and Molecular Therapeutics,The Children's Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,The Children's Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine,University of Pennsylvania

Journal (Genetics)

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Hellen E. Ahrens*¹, Henriette Henze*¹, Svenja C. Schüler¹, Manuel Schmidt¹, Sören S. Hüttner¹, Julia von Maltzahn¹

¹Leibniz-Institute on Aging - Fritz-Lipmann-Institute

Journal (Genetics)

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Mohamed A. Sobhy*¹, Amer Bralić*¹, Vlad-Stefan Raducanu¹, Muhammad Tehseen¹, Yujing Ouyang¹, Masateru Takahashi¹, Fahad Rashid¹, Manal S. Zaher¹, Samir M. Hamdan¹

¹Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST)

Journal (Genetics)

Improving Small RNA-seq: Less Bias and Better Detection of 2′-O-Methyl RNAs

Erwin L. van Dijk¹, Evangelia Eleftheriou¹, Claude Thermes¹

¹Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud,Université Paris-Saclay

Journal (Genetics)

A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

Huilin Wang*¹, Xiaofan Zhu*^2,3, Baoheng Gui*^3,4, Wan Chee Cheung², Mengmeng Shi^2,3, Zhenjun Yang^2,3, Ka Yin Kwok², Ricky Lim⁵, Sanna Pietilä⁵, Yuanfang Zhu^6,7, Kwong Wai Choy^2,3

¹Central Laboratory, Bao'an Maternity and Child Health Hospital,Jinan University, ²Department of Obstetrics and Gynaecology,The Chinese University of Hong Kong, ³Shenzhen Research Institute,The Chinese University of Hong Kong, ⁴Department of Genetics and Metabolism,Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, ⁵PerkinElmer Diagnostics, ⁶Department of Obstetrics and Gynecology, Bao'an Maternity and Child Health Hospital,Jinan University, ⁷Maternal-Fetal Medicine Institute, Bao'an Maternity and Child Health Hospital,Jinan University

Journal (Genetics)

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

Francesca Boscolo Sesillo^1,2,3, Alessandra Sacco²

¹Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, ²Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, ³Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego

Journal (Genetics)

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands

Clément Immarigeon¹, François Karch¹, Robert K. Maeda¹

¹Department of Genetics and Evolution,University of Geneva

Journal (Genetics)

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Katherine Chan*¹, Amy Hin Yan Tong*¹, Kevin R Brown¹, Patricia Mero¹, Jason Moffat^1,2,3

¹Donnelly Centre,University of Toronto, ²Department of Molecular Genetics,University of Toronto, ³Institute for Biomaterials and Biomedical Engineering,University of Toronto

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