JOVE JOURNAL

Developmental Biology

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Journal (Developmental Biology)

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Daniel Prieto¹, Gonzalo Aparicio^1,2, Matías Machado¹, Flavio R. Zolessi^1,2

¹Institut Pasteur de Montevideo, ²Sección Biología Celular, Facultad de Ciencias,Universidad de la República

Journal (Developmental Biology)

Immunostaining to Visualize Murine Enteric Nervous System Development

Amanda J. Barlow-Anacker¹, Christopher S. Erickson¹, Miles L. Epstein², Ankush Gosain^1,2,3

¹Department of Surgery,University of Wisconsin School of Medicine and Public Health, ²Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, ³Department of Pediatrics,University of Wisconsin School of Medicine and Public Health

Journal (Developmental Biology)

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies

Daniel Vogt¹, Pei-Rung Wu¹, Shawn F. Sorrells², Christine Arnold², Arturo Alvarez-Buylla², John L. R. Rubenstein¹

¹Department of Psychiatry,University of California San Francisco, ²Department of Neurological Surgery,University of California San Francisco

Journal (Developmental Biology)

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Philipp Schlaefli*¹, Alessandro Bertolo*¹, Cherry Malonzo¹, Tobias Poetzel², Martin Baur³, Frank Steffen⁴, Jivko Stoyanov¹

¹Swiss Paraplegic Research, ²Orthopaedics and Spinal Surgery,Swiss Paraplegic Centre, ³Department of Neurosurgery,Lucerne Cantonal Hospital (LUKS), ⁴Section of Small Animal Surgery/Neurology,Vetsuisse Faculty, University of Zurich

Journal (Developmental Biology)

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Lara Stevanato¹, Caroline Hicks¹, John D. Sinden¹

¹ReNeuron

Journal (Developmental Biology)

Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes

Adam C. Vandergriff^1,2, Michael Taylor Hensley^1,2, Ke Cheng^1,2,3

¹Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research,College of Veterinary Medicine, North Carolina State University, ²Joint Department of Biomedical Engineering,University of North Carolina at Chapel Hill, North Carolina State University, ³The Cyrus Tang Hematology Center,Soochow University

Journal (Developmental Biology)

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Sandra Zehentmeier¹, Zoltan Cseresnyes^2,3, Juan Escribano Navarro⁴, Raluca A. Niesner², Anja E. Hauser^1,5

¹Immunodynamics,German Rheumatism Research Center, a Leibniz Institute, ²Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute, ³Max-Delbrück Center for Molecular Medicine, ⁴Wimasis GmbH, ⁵Immunodynamics and Intravital Imaging,Charité - University of Medicine

Journal (Developmental Biology)

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Choong Yong Ung¹, Feng Guo², Xiaoling Zhang³, Zhihui Zhu³, Shizhen Zhu^1,3

¹Department of Molecular Pharmacology & Experimental Therapeutics,Mayo Clinic College of Medicine, Center for Individualized Medicine, ²Tufts University School of Medicine, ³Department of Biochemistry and Molecular Biology,Mayo Clinic

Journal (Developmental Biology)

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Lisa D. Wilsbacher^1,2, Shaun R. Coughlin²

¹Feinberg Cardiovascular Research Institute,Northwestern University, ²Cardiovascular Research Institute,University of California, San Francisco

Journal (Developmental Biology)

A Time Differential Staining Technique Coupled with Full Bilateral Gill Denervation to Study Ionocytes in Fish

Velislava Tzaneva¹, Steve F. Perry¹

¹Department of Biology,University of Ottawa

Journal (Developmental Biology)

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Arun Sharma*¹, Guang Li*¹, Kuppusamy Rajarajan*¹, Ryoko Hamaguchi¹, Paul W. Burridge¹, Sean M. Wu^1,2

¹Stanford Cardiovascular Institute,Stanford University School of Medicine, ²Institute of Stem Cell Biology and Regenerative Medicine, Cardiovascular Medicine Division, Department of Medicine, Child Health Research Institute,Stanford University School of Medicine

Journal (Developmental Biology)

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles

Stephanie Morgan¹, Lisa Campbell¹, Vivian Allison¹, Alison Murray², Norah Spears¹

¹Centre for Integrative Physiology,University of Edinburgh, ²MRC Centre for Reproductive Health,University of Edinburgh

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