Using Avian Skin Explants to Study Tissue Patterning and Organogenesis

Tingxin Jiang; Maeve Secor; Rusty Lansford; Randall B. Widelitz; Cheng Ming Chuong

doi:10.3791/65580

Journal

Developmental Biology

A Comprehensive Review of Avian Embryonic Skin Explant Culture Protocols

September 15, 2023

Author Spotlight: Insight into the Current Experimental Avian Skin Explant Methodologies

02:28 min

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554 Views

Chicken Skin Explant Culture for Organogenesis Study

02:15 min

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92 Views

Chicken Skin Epithelial-Mesenchymal Recombination Culture

02:17 min

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56 Views

Tissue Studies by Culturing Reconstituted Chicken Skin

02:39 min

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59 Views

Tingxin Jiang¹, Maeve Secor², Rusty Lansford³, Randall B. Widelitz¹, Cheng Ming Chuong¹

¹Department of Pathology,Keck School of Medicine of University of Southern California, ²Molecular and Computational Biology,University of Southern California, ³Department of Radiology,Keck School of Medicine of University of Southern California

Summary

Here we describe protocols for three types of avian embryonic skin explant cultures that can be used to examine tissue interactions, 4D imaging timelapse movie (3D plus time), global or local perturbation of molecular function, and systems biology characterization.